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This article in

  1. Vol. 86 No. 6, p. 1291-1295
    Received: Aug 06, 2007
    Accepted: Feb 15, 2008
    Published: December 5, 2014

    2 Corresponding author(s):


Technical note: A novel method for routine genotyping of horse coat color gene polymorphisms1

  1. L. J. Royo*,
  2. I. Fernández*,
  3. P. J. Azor,
  4. I. Álvarez*,
  5. L. Pérez-Pardal* and
  6. F. Goyache*2
  1. Servicio Regional de Investigación y Desarrollo Agroalimentario del Principado de Asturias-Somió, C/Camino de los Claveles 604, E-33203 Gijón (Asturias), Spain; and
    Departamento de Genética, Universidad de Córdoba, Campus de Rabanales, E-14071, Córdoba, Spain


The aim of this note is to describe a reliable, fast, and cost-effective real-time PCR method for routine genotyping of mutations responsible for most coat color variation in horses. The melanocortin-1 receptor, Agouti-signaling peptide, and membrane-associated transporter protein alleles were simultaneously determined using 2 PCR protocols. The assay described here is an alternative method for routine genotyping of a defined number of polymorphisms. Allelic variants are detected in real time and no post-PCR manipulations are required, therefore limiting costs and possible carryover contamination. Data can be copied to a Microsoft Excel spreadsheet for semiautomatic determination of the genotype using a macro freely available at (last accessed February 26, 2007). The performance of the method is demonstrated on 156 Spanish Purebred horses.

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