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Journal of Animal Science Abstract - Nonruminant Nutrition

Pediocin A modulates intestinal microflora metabolism in swine in vitro intestinal fermentations

 

This article in

  1. Vol. 87 No. 6, p. 2020-2028
     
    Received: Aug 28, 2008
    Accepted: Feb 16, 2009
    Published: December 5, 2014


    3 Corresponding author(s): andrea.piva@unibo.it
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doi:10.2527/jas.2008-1438
  1. G. Casadei12,
  2. E. Grilli and
  3. A. Piva32
  1. Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, 40064, Bologna, Italy

Abstract

The aim of this study was to investigate in vitro the effects of pediocin A [a bacteriocin produced by lactic acid bacteria (LAB) Pediococcus pentosaceus FBB61] on microbial metabolism in the small and large intestine of pigs. Pediocin A was partially purified by ion exchange chromatography and added to an in vitro fermentation system. The intestinal inoculum was collected from pigs immediately after slaughter, diluted with a buffer, and dispensed into fermentation syringes and vessels of the 2 experimental groups: 1) Bac+ = cecal liquor + predigested diet + pediocin A (final concentration 160 activity units/mL); 2) Bac− = cecal liquor + predigested diet + partially purified supernatant of P. pentosaceus FBB61–2. Intestinal microbial growth was monitored using the cumulative gas production technique; the kinetics of fermentation, bacterial counts, VFA, ammonia, polyamines, and p-cresol production were analyzed. Pediocin A had almost no effects on small intestine fermentation parameters, whereas in the cecum pediocin A decreased gas production (−16%; P < 0.05), ammonia, and VFA production (−52 and −21%, respectively, after 24 h; P < 0.001) compared with the control group. Significant inhibition of clostridia and LAB occurred in cecal fermentations: the Bac+ group yielded a decreased number of clostridia and LAB in cecal fermentations (8.19 and 7.80 cfu/mL, respectively) compared with Bac− (9.32 and 8.95 cfu/mL, respectively; P < 0.001). The low clostridia counts in the pediocin-treated group may also explain the reduced concentration of the carcinogenic compound p-cresol (−88%; P < 0.01). Our results suggest that pediocin A could be an alternative to replace antibiotic growth promoters for the prophylaxis of enteric diseases and to improve production of farm animals.

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