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Journal of Animal Science Abstract - Animal Nutrition

Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets1

 

This article in JAS

  1. Vol. 91 No. 6, p. 2740-2748
     
    Received: Aug 29, 2012
    Accepted: Feb 19, 2013
    Published: November 25, 2014


    2 Corresponding author(s): yinyulong@isa.ac.cn
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doi:10.2527/jas.2012-5795
  1. H. S. Yang*†,
  2. D. Z. Fu*†,
  3. X. F. Kong*‡,
  4. W. C. Wang*†,
  5. X. J. Yang§,
  6. C. M. Nyachoti# and
  7. Y. L. Yin 2
  1. Chinese Acad. Sci., Inst. Subtrop. Agric., Res. Ctr. Healthy Breeding Livestock & Poultry, Hunan Eng. & Res. Ctr. Anim. & Poultry Sci., Key Lab Agroecol. Proc. Subtrop. Reg., Scientific Observation and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha City, Hunan, 410125, P. R. China
    University of Chinese Academy of Sciences, Beijing, 100049, P. R. China
    Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang, Guangxi, 547100, P. R. China
    Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario N1G 5C9, Canada
    Department of Animal Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada

Abstract

Weaning is associated with reduced intestinal absorptive capacity in piglets. Our previous study indicated that dietary supplementation with N-carbamylglutamate (NCG) enhanced growth performance and improved intestinal function in weaned piglets. The present study was conducted to test the hypothesis that dietary supplementation with NCG may increase the growth performance of weaned piglets by regulating the expression of intestinal nutrient transporters, thus enhancing nutrient absorption. Twenty-four Huanjiang mini-pig piglets weaned at 21 d of age (3.17 ± 0.21 kg average BW) were randomly assigned to 2 dietary treatments consisting of a basal diet and the basal diet with 0.1% NCG supplementation for a 14-d period with 6 pens per treatment and 1 male and 1 female per pen. On d 14, 1 piglet was randomly selected from each pen for blood and tissue sampling. Dietary NCG supplementation enhanced (P < 0.05) growth rate and the efficiency of feed use in weaned Huanjiang mini-pig piglets. The NCG-supplemented diet increased (P < 0.05) mRNA expression levels of Slc6a19, Slc7a9, and Slc1a1 and the protein abundance of ASCT2, B0AT1, b0,+AT, y+LAT1, and EAAC1 in the jejunum. Furthermore, the contents of low density lipoprotein, ammonia, urea nitrogen, and AA as well as the activity of alkaline phosphatase in plasma were all altered (P < 0.05) by supplementation with NCG. These findings indicate that dietary supplementation with NCG may improve intestinal absorptive function in weaned piglets by increasing the expression of AA transporters in the intestine.

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