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Journal of Animal Science Abstract - Animal Physiology

Impact of oral meloxicam on circulating physiological biomarkers of stress and inflammation in beef steers after long-distance transportation1

 

This article in JAS

  1. Vol. 92 No. 2, p. 498-510
     
    Received: July 03, 2013
    Accepted: Nov 24, 2013
    Published: November 24, 2014


    2 Corresponding author(s): hcoetzee@iastate.edu
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doi:10.2527/jas.2013-6857
  1. N. K. Van Engen*,
  2. M. L. Stock,
  3. T. Engelken*,
  4. R. C. Vann,
  5. L. W. Wulf*,
  6. L. A. Karriker*,
  7. W. D. Busby§,
  8. J. Lakritz#,
  9. A. J. Carpenter,
  10. B. J. Bradford,
  11. W. H. Hsu,
  12. C. Wang* and
  13. J. F. Coetzee 2
  1. Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011;
    Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames 50011;
    Brown Loam Branch Experiment Station, Mississippi Agricultural Forestry and Experiment Station, Mississippi State University, Raymond 39154;
    Tri-County Steer Carcass Futurity Cooperative, Lewis, IA 51544;
    Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210;
    Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan 66506

Abstract

Transportation stress can result in significant economic losses to producers due to decreased animal productivity and increased medication costs associated with sickness such as bovine respiratory disease (BRD). Meloxicam (MEL) provides pain relief and anti-inflammatory effects in cattle for several days after a single oral treatment. Our hypothesis was that MEL administration before shipping would reduce the impact of long-distance transportation on circulating physiological biomarkers of stress and inflammation in beef steers. Ninety-seven beef steers were blood sampled for baseline biomarker determination and then randomly assigned to receive either 1 mg/kg MEL (n = 49) or a placebo (CONT; n = 48) per os before a 1,316-km transportation event lasting approximately 16 h. Calves were then blood sampled on arrival and 5 d later. Changes in the hemogram, circulating plasma proteins, total carbon dioxide (TCO2), fibrinogen, substance P (SP), cortisol, haptoglobin (Hp)-matrix metalloproteinase-9 (MMP-9) complexes, and tumor necrosis factor α (TNFα) between treatments over time were compared using a mixed effects model with statistical significance designated as P < 0.05. Analysis of covariance was conducted to assess the relationship between circulating MEL concentrations and biomarker changes over time. An increase in neutrophil, platelet, monocyte, white blood cell, and red blood cell counts occurred after transportation (P < 0.0001) and a decrease in lymphocyte count were observed (P < 0.0001). Meloxicam treatment reduced the stress-induced neutrophilia (P = 0.0072) and circulating monocyte count (P = 0.013) on arrival. Mean corpuscle hemoglobin (P = 0.05), mean corpuscle volume (P = 0.05), and lymphocyte count (P = 0.05) were also greater in the CONT calves compared with MEL calves after transportation. Furthermore, Hp-MMP-9 complexes, TCO2, TNFα, plasma proteins, and SP increased and cortisol decreased after shipping (P < 0.01). Meloxicam treatment tended to reduce serum cortisol concentrations (P = 0.08) and there was evidence of a time × treatment interaction (P = 0.04). An inverse relationship between plasma MEL concentrations and circulation cortisol concentrations (P = 0.002) and neutrophil (P = 0.04) and basophil counts (P = 0.03) was also observed. The results suggest that MEL administration may reduce the impact of long-distance transportation on circulating physiological biomarkers of stress and inflammation in beef calves.

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