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Journal of Animal Science Abstract - Nonruminant Nutrition

Effects of a 3 strain Bacillus-based direct-fed microbial and dietary fiber concentration on growth performance and expression of genes related to absorption and metabolism of volatile fatty acids in weanling pigs1

 

This article in JAS

  1. Vol. 95 No. 1, p. 308-319
     
    Received: Aug 15, 2016
    Accepted: Oct 24, 2016
    Published: February 2, 2017


    3 Corresponding author(s): hstein@illinois.edu
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doi:10.2527/jas2016.0557
  1. N. W. Jaworski*,
  2. A. Owusu-Asiedu22,
  3. M. C. Walsh,
  4. J. C. McCann*,
  5. J. J. Loor* and
  6. H. H. Stein 3*‡
  1. * Department of Animal Sciences, University of Illinois, Urbana 61801
     Danisco Animal Nutrition– DuPont Industrial Biosciences, Marlborough, UK SN8 1XN
     Division of Nutritional Sciences, University of Illinois, Urbana 61801

Abstract

Effects of a Bacillus-based direct-fed microbial (DFM) on growth performance, plasma tumor necrosis factor ɑ (TNF-ɑ), relative gene expression, and intestinal VFA concentrations in weanling pigs fed low- or high-fiber diets were evaluated. Two hundred pigs (initial BW: 6.31 ± 0.73 kg) were allotted to 1 of 4 dietary treatments (5 pigs per pen and 10 pens per treatment). Treatments were arranged in a 2 × 2 factorial design with 2 diet types [low-fiber (LF) or high-fiber (HF)] and 2 concentrations of DFM (0 or 60 g DFM/t of feed). The DFM contained 1.5 × 105 cfu/g and was obtained from Danisco Animal Nutrition-DuPont Industrial Biosciences, Marlborough, UK. Phase 1 diets were fed for 2 wk post-weaning and phase 2 diets were fed over the following 29 d. Low fiber diets contained corn and soybean meal as main ingredients and HF diets contained corn, soybean meal, corn distillers dried grains with solubles (7.5 and 15.0% in phase 1 and 2, respectively), and wheat middlings (10.0%). Pigs and feed were weighed at the start and at the end of each phase, and ADG, ADFI, and G:F were calculated. At the conclusion of phase 2, blood was collected from 1 pig per pen and 1 pig per pen was sacrificed. Cecum and rectum contents were analyzed for VFA, and tissue samples were collected from the ileum, cecum, rectum, and liver to determine expression of genes related to absorption and metabolism of VFA using quantitative reverse transcription-PCR. Results indicated that feeding HF diets reduced (P ≤ 0.05) ADFI and ADG of pigs compared with feeding LF diets. Pigs fed DFM diets had improved (P ≤ 0.05) G:F compared with pigs fed non-DFM diets. Pigs fed LF diets had greater (P ≤ 0.05) BW at the end of phase 2 compared with pigs fed HF diets. The concentration of VFA in rectum contents was greater (P ≤ 0.05) in pigs fed LF diets than in pigs fed HF diets. The expression of monocarboxylate transporter-1 in the rectum of pigs fed HF diets was greater (P ≤ 0.05) than for pigs fed LF diets, and pigs fed DFM-containing diets had an increased (P ≤ 0.05) expression of glucagon-like peptide-2 receptor in the liver. Pigs fed HF diets had greater (P ≤ 0.05) concentrations of urea N in plasma compared with pigs fed LF diets, but dietary fiber and DFM had no effect on plasma concentration of TNF-ɑ. In conclusion, the Bacillus–based DFM improved overall G:F of weanling pigs, but pigs fed LF diets had greater final BW than pigs fed HF diets.

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