Differentiation of stromal-vascular cells into adipocytes. Stromal-vascular cells were induced to differentiate using differentiation media (Diff 1, 1.1, 2, 3) or grown in medium without differentiation components [Dulbecco’s modified Eagle’s medium (DMEM), Sigma-Aldrich, St. Louis, MO]. Differentiation measurements were executed on d 8 after initiation of differentiation and included incorporation of radiolabeled acetate into lipids (A) and activity of glycerol-3-phosphate dehydrogenase (G3PDH; B). The mRNA abundance of fatty acid-binding protein 4 (aP2; C), PPARγ (D), and acetyl-CoA carboxylase-α (ACCα; E) was measured by real-time PCR, with DMEM serving as the comparator. Error bars are SEM. Means lacking a common letter (a–c) are different (P < 0.05). This experiment was repeated 3 times with cells isolated from different animals, and duplicate wells were used for each measurement (n = 6).