View Full Table | Close Full ViewTable 1.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb BW and visceral organ mass1,2

 
Se supply3
Nutritional plane4
P-value5
Item ASe HSe SEM6 RES CON HIH SEM7 Se Nut Se by Nut
BW, kg 10.8 11.1 0.4 9.9a 11.2b 11.6b 0.4 0.57 0.02 0.51
Empty BW,8 kg 9.6 9.9 0.3 8.9a 10.1b 10.3b 0.4 0.39 0.03 0.46
Empty gastrointestinal tract, g 541 553 17 505a 562b 573b 21 0.60 0.05 0.75
Stomach complex, g 121 118 3 109a 126b 123b 4 0.50 0.005 0.31
Small intestine, g 337 345 13 318 345 360 17 0.67 0.18 0.68
Large intestine, g 0.53 0.03 0.03
    ASe 82.4y 84.2y 81.9xy 4.5
    HSe 71.2x 90.0yz 94.2z 5.0
Liver, g 301 304 11 278a 316b 315b 13 0.85 0.06 0.17
Pancreas, g 0.60 0.65 0.02
    ASe 12.7x 13.0x 14.7y 0.7
    HSe 13.8xy 14.8y 12.8x 0.8
a,bWithin an item, main effect means differ (P < 0.10).
x-zWithin an item, interactive means differ (P < 0.10).
1Lambs were raised independently of their dams.
2Portions of these data were previously published in Meyer et al. (2009) and Camacho et al. (2012).
3Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
4Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
5P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
6SEM for ASe (n = 39) and HSe (n = 36).
7SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).
8Empty BW = BW – digesta weight.



View Full Table | Close Full ViewTable 2.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb visceral organ proportional mass (g/kg empty BW)1,2

 
Se supply3
Nutritional plane4
P-value5
Item ASe HSe SEM6 RES CON HIH SEM7 Se Nut Se by Nut
Empty gastrointestinal tract 56.6 56.4 1.0 57.5 56.5 55.5 1.3 0.86 0.53 0.27
Stomach complex 0.22 0.58 0.07
    ASe 12.0x 13.9y 12.8xy 0.7
    HSe 13.1xy 11.9x 11.6x 0.8
Small intestine 35.2 35.6 0.8 36.2 35.1 34.9 1.0 0.73 0.63 0.36
Large intestine 0.70 0.78 0.04
    ASe 9.27y 8.62xy 7.85x 0.45
    HSe 8.34xy 8.66xy 9.14y 0.47
Liver 0.52 0.26 0.08
    ASe 32.8y 30.4x 29.9x 1.0
    HSe 29.9x 31.9xy 29.9x 1.0
Pancreas 1.45 1.43 0.06 1.56b 1.42ab 1.33a 0.08 0.80 0.10 0.14
a,bWithin an item, main effect means differ (P < 0.10).
x,yWithin an item, interactive means differ (P < 0.10).
1Lambs were raised independently of their dams.
2Portions of these data were previously published in Camacho et al. (2012).
3Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
4Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
5P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
6SEM for ASe (n = 39) and HSe (n = 36).
7SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).



View Full Table | Close Full ViewTable 3.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb jejunal mucosal cellularity and density1

 
Se supply2
Nutritional plane3
P-value4
Item ASe HSe SEM5 RES CON HIH SEM6 Se Nut Se by Nut
DNA, mg/g 5.40 5.38 0.15 5.17a 5.30a 5.71b 0.18 0.93 0.07 0.15
DNA, g7 1.53 1.57 0.08 1.38a 1.56ab 1.72b 0.09 0.67 0.04 0.26
RNA, mg/g 5.55 5.45 0.18 5.33 5.50 5.68 0.22 0.71 0.54 0.11
RNA, g7 1.59 1.59 0.09 1.43 1.61 1.72 0.12 0.97 0.21 0.14
Protein, mg/g 28.3 27.3 1.7 27.1 27.8 28.4 2.1 0.65 0.90 0.95
Protein, g7 8.35 8.16 0.71 7.52 8.42 8.83 0.90 0.85 0.56 0.83
RNA:DNA 1.03 1.03 0.02 1.04 1.06 1.00 0.03 0.90 0.29 0.12
Protein:DNA 5.37 5.15 0.32 5.31 5.40 5.06 0.40 0.62 0.80 0.80
Mucosal density, % 83.4 83.6 0.5 83.6 83.8 83.0 0.6 0.79 0.55 0.12
a,bWithin an item, main effect means differ (P < 0.10).
1Lambs were raised independently of their dams.
2Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
3Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
4P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
5SEM for ASe (n = 39) and HSe (n = 36).
6SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).
7Total small intestinal mucosal DNA, RNA, and protein content (small intestinal mass × mucosal density × cellularity concentration).



View Full Table | Close Full ViewTable 4.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb jejunal proliferation and villus morphology1

 
Se supply2
Nutritional plane3
P-Value4
Item ASe HSe SEM5 RES CON HIH SEM6 Se Nut Se by Nut
Proliferation, % 0.37 0.72 0.003
    ASe 5.26xy 3.78x 7.35z 0.86
    HSe 5.15xy 5.80yz 3.56x 0.94
Total small intestinal cells, × 1012 278 285 14 250a 280ab 314b 17 0.69 0.03 0.27
Total proliferating small intestinal cells, × 1012 0.34 0.16 <0.001
    ASe 11.8x 10.2x 23.3y 2.5
    HSe 14.4x 14.9x 10.4x 2.7
Crypt depth, µm 60.8 61.5 1.7 58.4 61.9 63.1 2.1 0.78 0.25 0.76
Villus length, µm 271 278 8 261 285 277 10 0.53 0.17 0.45
a,bWithin an item, main effect means differ (P < 0.10).
x−zWithin an item, interactive means differ (P < 0.10).
1Lambs were raised independently of their dams.
2Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
3Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
4P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
5SEM for ASe (n = 39) and HSe (n = 36).
6SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).



View Full Table | Close Full ViewTable 5.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb jejunal mucosal angiogenic factor and glucagon like peptide-2 mRNA expression (gene of interest/18S)1,2

 
Se supply3
Nutritional plane4
P-values5
Item6 ASe HSe SEM7 RES CON HIH SEM8 Se Nut Se by Nut
VEGF 0.184 0.210 0.013 0.199 0.198 0.194 0.017 0.16 0.97 0.60
FLT1 0.030 0.030 0.002 0.031 0.030 0.031 0.002 0.97 0.97 0.83
KDR 0.098 0.105 0.010 0.104 0.097 0.103 0.012 0.59 0.91 0.90
NOS3 0.399 0.380 0.043 0.373 0.455 0.340 0.052 0.74 0.25 0.24
GUCY1B3 0.11 0.22 0.07
    ASe 0.138xy 0.228yz 0.300z 0.042
    HSe 0.199xyz 0.126x 0.172xy 0.047
GLP-2 0.393 0.472 0.043 0.537b 0.359a 0.401a 0.053 0.19 0.05 0.62
GLP-2R 0.771 0.898 0.050 0.799 0.794 0.880 0.063 0.13 0.53 0.92
a,bWithin an item, main effect means differ (P < 0.10).
x–zWithin an item, interactive means differ (P < 0.10).
1Lambs were raised independently of their dams.
2GLP-2 and GLP-2R data were previously published in Caton et al. (2009b).
3Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
4Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
5P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
6VEGF = vascular endothelial growth factor, FLT1 = VEGF receptor-1, KDR = VEGF receptor-2, NOS3 = endothelial nitric oxide synthase 3, GUCY1B3 = soluble guanylate cyclase, GLP-2 = glucagon-like peptide-2, GLP-2R = GLP-2 receptor.
7SEM for ASe (n = 39) and HSe (n = 36).
8SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).



View Full Table | Close Full ViewTable 6.

Effects of maternal Se supply and nutritional plane during gestation on neonatal lamb jejunal vascularity1

 
Se supply2
Nutritional plane4
P-values5
Item ASe HSe SEM3 RES CON HIH SEM6 Se Nut Se by Nut
Capillary area density, % 20.6 21.0 0.6 20.5 21.7 20.1 0.7 0.61 0.20 0.59
Capillary no. density, per mm2 1,134 1,139 46 1,092 1,149 1,171 56 0.93 0.59 0.33
Capillary surface density, (µm/µm2) × 10 0.98 1.00 0.03 0.93a 1.05b 0.99ab 0.03 0.53 0.05 0.78
Area per capillary, µm2 0.60 0.37 0.09
    ASe 240z 188xy 179x 20
    HSe 197xyz 235yz 202xyz 22
Total small intestinal vascularity, mL 68.7 73.6 3.1 65.0 76.5 72.0 3.9 0.27 0.11 0.66
a,bWithin an item, main effect means differ (P < 0.10).
x–zWithin an item, interactive means differ (P < 0.10).
1Lambs were raised independently of their dams.
2Lambs born to ewes fed 11.5 µg of Se/kg of BW (adequate Se, ASe) or 77.0 µg of Se/kg of BW (high Se, HSe) from breeding to parturition.
3SEM for ASe (n = 39) and HSe (n = 36).
4Lambs born to ewes fed 60% (restricted, RES), 100% (control, CON), or 140% (high, HIH) of nutrient requirements from d 40 of gestation to parturition.
5P-values for Se supply (Se), nutritional plane (Nut), and their interaction.
6SEM for RES (n = 23), CON (n = 27), and HIH (n = 25).