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Primer sequences for real-time reverse transcription PCR and source references

 
Gene1 Primer2 Sequence Source
AMH F 5′-AAGGTGGTCAAGTCACTCAG-3′ Ireland et al. (2009)
R 5′-CAGGGAAGAAGTCTTCAGCA-3′
GAPDH F 5′-GGCGTGAACCACGAGAAGTAT-3′ Klipper et al. (2004)
R 5′-CGTGGACAGTGGTCATAAGT-3′
PTX3 F 5′-TCTTTATTTATCTTGGCAAAAT-ACTGAGTAA-3′ Chitko-McKown et al. (2004)
R 5′-AAGCACCATGGCATAAAATCT-AGTAA-3′
1AMH = anti-Müllerian hormone; GADPH = glyceraldehydes-3-phosphate dehydrogenase; PTX3 = pentraxin 3.
2F = forward primer; R = reverse primer.



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Comparisons of BW between repeat breeder cows and control cows

 
Weights Repeat breeder Control P-value
Cows, n 64 72
Birth weight, kg 38.4 ± 0.6 37.3 ± 0.6 0.23
Weaning weight, kg 217.1 ± 3.6 222.4 ± 3.4 0.29
Weight on study, kg 671.4 ± 9.0 642.8 ± 8.5 0.02



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Comparisons of production traits between repeat breeder cows and control cows

 
Performance trait Repeat breeder Control P-value
Cows, n 64 72
Age at first breeding, d 426.5 ± 2.1 425.5 ± 2.3 0.74
Age at first calving, d 758.8 ± 11.3 715.9 ± 10.6 0.006
Age at last calving, d 1710.9 ± 103.4 3467.2 ± 97.5 <0.0001
Days postpartum 475.6 ± 18.9 249.1 ± 17.8 <0.0001
Days postweaning 325.24 ± 20.1 115.2 ± 18.9 <0.0001



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Comparisons of reproductive organ measurements between repeat breeder cows and control cows

 
Organ measurements Repeat breeder Control P-value
Cows, n 64 72
Day of the estrous cycle 5.9 ± 0.2 5.7 ± 0.2 0.44
Ovary length,1 mm 28.7 ± 1.8 34.7 ± 1.5 0.03
Ovary height,2 mm 18.2 ± 1.2 22.5 ± 1.0 0.02
Endometrial diameter,3 mm 11.5 ± 1.1 18.9 ± 1.0 <0.0001
1Length of the ovary contralateral to the corpus luteum.
2Height of the ovary contralateral to the corpus luteum.
3The distance from the myometrium interface on 1 side to the opposite myometrium interface measured 1 to 2 cm anterior to the uterine bifurcation.