Figure 1.
Figure 1.

Distribution of final BW of rainbow trout, O. mykiss, fingerlings fed the control diet (commercial diet, Aller Futura; Aller Aqua, Christiansfeld, Denmark) or probiotic diet (commercial diet containing 2 × 104 cfu/g of B. cereus var. toyoi). Mean frequency values for each size category are based on 3 replicate tanks with 120 to 125 fish per tank. Within a BW range, means for control and probiotic diets with asterisks differ (P < 0.01).

 


Figure 2.
Figure 2.

Distribution of Fulton’s condition factor (K) of rainbow trout, O. mykiss, fingerlings fed the control diet (commercial diet, Aller Futura; Aller Aqua, Christiansfeld, Denmark) or probiotic diet (commercial diet containing 2 × 104 cfu/g of B. cereus var. toyoi) diet. The K factor estimates the body condition of the fish and assumes that heavier fish of a given length are in better condition. Mean frequency values for each size category are based on 3 replicate tanks with 120 to 125 fish per tank. Within a BW range, means for control and probiotic diets with asterisks differ (P < 0.01).

 


Figure 3.
Figure 3.

Box and whisker plots of the villus width and height and intestinal goblet cell number of rainbow trout, O. mykiss, fingerlings fed the control diet (commercial diet, Aller Futura; Aller Aqua, Christiansfeld, Denmark) or probiotic diet (commercial diet containing 2 × 104 cfu/g of B. cereus var. toyoi). Measurements of total goblet cell number (full and empty) and villi height were based on the analysis of 8 randomly chosen fields from the intestinal mucosa of 12 fish per diet (3 replicate pens/diet). The asterisk denotes that means for the control and probiotic diets differ (P < 0.01). Solid and dashed lines represent the median and mean values for each set of samples, respectively, whereas dots represent the outliers.

 


Figure 4.
Figure 4.

Percentage of similarity among PCR-restriction fragment length polymorphism band patterns in the posterior intestine samples (5/diet) from rainbow trout, O. mykiss, fingerlings fed the control diet (commercial diet, Aller Futura; Aller Aqua, Christiansfeld, Denmark) or probiotic diet (T; commercial diet containing 2 × 104 cfu/g of B. cereus var. toyoi). R1 to R5 correspond to replicate numbers.