Figure 1.
Figure 1.

Relationship between the calculated acidosis index and values predicted using the first near-infrared reflectance (NIR) spectroscopy calibration, which was based on NIR spectroscopy of whole grain scans. The fine solid line represents the line of equivalence and the dotted lines represent the SE of cross-validation ± 8.46% units. The heavier solid line is the linear regression (y = 0.96x + 1.83).

 


Figure 2.
Figure 2.

Mean rumen A) valerate concentration, B) propionate concentration, and C) ammonia concentration from cattle (n = 16) fed 0, 0.4, 0.8 or 1.2% of BW of mixed grain or triticale at 5 time periods after diet consumption in the method development study.

 


Figure 3.
Figure 3.

Mean rumen A) pH, B) propionate concentration, C) valerate concentration, and D) ammonia concentration for 20 grain cultivars and control over 5 time periods, 5, 65, 110, 155, and 200 min, after diet consumption in the in vivo study.Barley1 (Tantangara), Barley2 (Gairdner 3864), Barley3 (Gairdner 3862), Barley4 (Binnalong); Oats1 (Dalyup 5817), Oats2 (Dalyup 5818), Oats3 (Swan); Sorghum1 (Liberty), Sorghum2 (Pacer), Sorghum3 (MR43); Triticale1 (Maiden), Triticale2 (Jackie 6823), Triticale3 (Jackie 6824), Triticale3 (Prime 322); Wheat1 (Bellaroi), Wheat2 (Sentinal), Wheat3 (Ammrock), Wheat4 (Kellalac), Wheat5 (Unknown), and Wheat6 (Chara). The control consisted of a blend of the 20 grain cultivars in equal proportions. See online version for figure in color.

 


Figure 4.
Figure 4.

Relationship between the calculated discriminant score and revised predicted acidosis index for the experimental grain cultivars. The line represents the linear regression (y = 0.0157 × x – 1.731; R2 = 0.69). NIR = near-infrared reflectance.