Figure 1.
Figure 1.

The mRNA expression of C/EBPα (A), C/EBPβ (B), PPARγ (C), and zip finger protein 423 (Zfp423) (D), and the intramuscular fat content (E) of Angus (■) and Wagyu (□) sternomandibularis muscle (*P < 0.05; n = 3; mean ± SE).

 


Figure 2.
Figure 2.

The mRNA expression of fibroblast growth factor (FGF)-2 (A), FGF receptor (FGFR) (B), fibronectin (C), and transforming growth factor (TGF)-β (D) in sternomandibularis muscle of Angus (■) and Wagyu (□; *P < 0.05; n = 3; mean ± SE).

 


Figure 3.
Figure 3.

The mRNA expression of Angus (■) and Wagyu (□) sternomandibularis muscle. Collagen I and III (A), collagen content (B), collagen solubility (C), and representative images of muscle stained with Masson’s trichrome (D and E) (*P < 0.05; n = 3; mean ± SE). See online version for figure in color.

 


Figure 4.
Figure 4.

Frequency and size distribution of muscle fibers from Wagyu (dash line) and Angus (solid line) (A), average muscle fiber diameter (B) and intercellular space (C), and the mRNA expression of MyoD and MyoG (D and E) (*P < 0.05; n = 3; mean ± SE).

 


Figure 5.
Figure 5.

Immunofluorescent staining of Angus and Wagyu sternomandibularis muscle. Fatty acid binding protein (FABP4) stained green and nuclei counterstained with DAPI (4’,6-diamidino-2-phenylindole) blue (A). Density of preadipocytes and adipocytes counted according to the positive staining for both FABP4 and DAPI (B) (*P < 0.05; n = 3; mean ± SE). See online version for figure in color.