Figure 1.
Figure 1.

Metabolic homeostasis is significantly altered in both our FGF21 transgenic (FGF21Tg) and FGF21 knockout (FGF21KO) mice. When fed either standard chow or high fat diet (HFD), our strains of FGF21Tg and FGF21KO mice exhibit opposing body weight phenotypes, with FGF21Tg mice displaying a lean phenotype while FGF21KO mice are mildly obese when compared with their wild-type counterparts. Concordant with these differing levels of adipose tissue, adiponectin secretion is elevated in our FGF21Tg mice and lowered in FGF21KO mice. See online version for figure in color.

 


Figure 2.
Figure 2.

FGF21 transgenic (FGF21Tg) mice exhibit improved glycemic control and enhanced sensitivity to the hormones insulin and leptin. Under fed conditions, FGF21Tg mice have lower basal levels of leptin (Panel A), insulin (Panel B), and serum glucose (Panel C). When challenged with an overnight fast FGF21Tg mice are able to maintain appropriate glucose levels (Panel C) despite extremely low levels of insulin (Panel B). Additionally, FGF21Tg mice are capable of proper leptin regulation when fasted (Panel A). Blood samples from fed and 24-h fasted 12 wk old, male wild-type and FGF21Tg mice (n = 6 per group) were collected on ice before storage of plasma at –80°C. Insulin (Crystal Chem Inc., Downers Grove, IL), leptin (Crystal Chem Inc.) and total adiponectin (BioVendor Inc., Karasek, Czech Republic) were measured by specific ELISA. Glucose was measured using a Hitachi 912 clinical chemistry analyzer (Roche Diagnostics, Indianapolis, IN).