Figure 1.
Figure 1.

Effect of fibroblast growth factor 9 (FGF9) on steroidogenesis in porcine granulosa cells (Exp. 1). Panel A: Effect of FGF9 (0, 3, 10, or 30 ng/mL) on estradiol production induced by FSH and FSH plus IGF-I (30 ng/mL) in cultured granulosa cells. Panel B: Effect of FGF9 (0, 3, 10, or 30 ng/mL) on progesterone production induced by FSH and FSH plus IGF-I (30 ng/mL) in cultured granulosa cells. All cells were concomitantly treated with 30 ng/mL of FSH and 500 ng/mL of testosterone. a,b,c,d,e,fWithin a panel, means (±SEM; n = 9) without a common letter differ (P < 0.05).

 


Figure 2.
Figure 2.

Effect of fibroblast growth factor 9 (FGF9; 0, 3, 10, or 30 ng/mL) on numbers of porcine granulosa cells treated with FSH and FSH plus IGF-I (30 ng/mL) in vitro (Exp. 1). All cells were concomitantly treated with 30 ng/mL of FSH and 500 ng/mL of testosterone. a,b,c,dMeans (±SEM; n = 9) without a common letter differ (P < 0.05).

 


Figure 3.
Figure 3.

Effect of FSH, IGF-I, and PGE2 (Exp. 2) and fibroblast growth factor 9 (FGF9; Exp. 5) on abundance of FGFR2IIIc mRNA in porcine granulosa cells. Panel A: Granulosa cells were treated with control (no additions), FSH (30 ng/mL), IGF-I (30 ng/mL), and/or PGE2 (300 ng/mL) in Exp. 2. Panel B: Granulosa cells were treated with FSH (30 ng/mL) and IGF-I (30 ng/mL) with and without FGF9 (30 ng/mL) in Exp. 5. Values are means of 3 replicate experiments and normalized to constitutively expressed 18S ribosomal RNA. a,bMeans (±SEM; n = 6) without a common superscript differ (P < 0.05).

 


Figure 4.
Figure 4.

Effect size of follicle on abundance of granulosa cell FGFR2IIIc mRNA in porcine follicles (Exp. 6). Granulosa cells from small (1 to 3 mm), medium (4 to 6 mm), and large (7 to 11 mm) follicles were collected, RNA isolated, and quantitative reverse transcription-PCR used to quantify mRNA levels. Values are normalized to constitutively expressed 18S ribosomal RNA. a,bWithin a panel, means (n = 6 to 8 pools per mean) without a common superscript differ (P < 0.05).