View Full Table | Close Full ViewTable 1.

Effect on nuclear maturation of the collection–maturation interval time

 
Experimental group1 No. of oocytes No. of mature oocyte, (%) No. of immature oocyte, (%) No. of degenerated oocyte, (%)
I: 4 h 136 76 (55, 9)2a 34 (25, 0) 26 (19, 1)a
II: 6 h 77 32 (41, 6)b 18 (23, 8) 27 (35, 0)b
III: 8 h 199 94 (47, 2)ab 43 (21, 6) 62 (31, 2)b
IV: 10 h 113 52 (46, 0)ab 24 (21, 2) 38 (33, 7)b
V: 12 h 83 32 (38, 6)b 25 (30, 1) 26 (31, 3)b
Total 608 286 (47.00) 144 (23.60) 179 (29.40)
a,bWithin a column, means without a common superscript differ (chi-square test, P < 0.05).
14, 6, 8, 10, and 12 h are hours of collection–maturation interval time.



View Full Table | Close Full ViewTable 2.

Effect of the collection–maturation interval time on equine cloned embryo development (activation protocol A)

 
Experimental group1 No. of oocytes No. of mature oocytes, (%) No. of ZFRE2 No. of cleaved, (%) No. of blastocysts, (%)
I: 4 to 6 h 135 73 (54.07)3a 53 36 (67.92)ab 3 (5.66)
II: 6 to 10 h 138 73 (52.89)ab 44 33 (75.00)a 1 (2.27)
III: 10 to 12 h 94 38 (40.42)b 27 11 (40.74)b 0 (0)
Total 367 184 (50.01) 108 80 (74.07) 4 (3.70)
a,bWithin a column, means without a common superscript differ (Fisher’s exact test, P < 0.05).
14 to 6, 6 to 10, and 10 to 12 h are hours of collection–maturation interval time.
2ZFRE = zona free reconstructed embryos.



View Full Table | Close Full ViewTable 3.

Effect of the collection–maturation interval time on equine cloned embryo development (activation protocol B)1

 
Experimental
group2
No. of oocytes No. of mature oocytes, (%) No. of ZFRE3 No. of cleaved, (%) No.of blastocysts, (%)
I: 4 a 7 h 56 33 (58.9)4 22 20 (90.9) 1 (4.5)
II: 7 a 10 h 111 49 (44.5) 34 28 (82.3) 7 (20.6)
Total 167 82 (49.1) 56 48 (85.7) 8 (14.3)
1No statistical differences were found between experimental groups (Fisher’s exact test, P < 0.05).
24 to 7 and 10 to 12 h are hours of collection–maturation interval time.
3ZFRE = zona free reconstructed embryos.



View Full Table | Close Full ViewTable 4.

Effect on oocyte nuclear maturation of pregnancy status of the mare donor of ovaries

 
Experimental
group
Collection rate (oocytes per ovary) No. of oocytes No. of mature oocytes, (%) No. of immature oocytes, (%) No. of degenerated oocytes, (%)
I: Nonpregnant 2.7a 329 177 (53.79)1a 39 (11.85) 113 (34.34)a
II: Pregnant 1.3b 146 53 (36.30)b 11 (7.53) 82 (56.16)b
Total 2 475 230 (48.42) 50 (10.52) 195 (41.05)
a,bWithin a column, means without a common superscript differ (chi-square test, P < 0.05).