View Full Table | Close Full ViewTable 1.

Composition of experimental diets, as-fed basis

 
Dietary composition1
Item Phase 1 Phase 2
Ingredient, %
    Yellow corn, ground 45.0 50.5
    Soybean meal, dehulled 23.9 27.9
    Dried whey 20.0 15.0
    Plasma protein 2.00 0.00
    Fish meal 5.00 3.00
    l-Lys HCl 0.10 0.10
    dl-Met 0.08 0.06
    l-Thr 0.02 0.00
    Salt 0.22 0.26
    Vitamin premix2 0.03 0.03
    Trace mineral premix3 0.15 0.15
    Dicalcium phosphate 1.15 0.80
    Ground limestone 0.25 0.60
    Poultry fat 2.00 1.5
Calculated composition
    DM, % 91.3 90.8
    ME, kcal/kg 3,269 3,313
    CP, % 22.4 21.3
    Lys, % 1.34 1.20
    Cys + Met, % 0.76 0.70
    Trp, % 0.25 0.23
    Thr, % 0.84 0.73
    Ca, % 0.91 0.82
    Available P, % 0.55 0.40
    Total P, % 0.82 0.68
Analyzed composition4
    DM, % 90.8 90.3
    CP, % 22.9 22.2
1Diets in each phase supplemented with 0.0, 0.2, 0.5, or 1.0 g/kg of a nucleotide additive (Inotide; CJ Corp, Seoul, Korea) for a total of 4 diets per phase. This additive contains 53% total nucleotides, with 51% of these as inosine 5′-monophosphate. The addition of the nucleotide additive replaced an equal amount of soybean meal in each diet.
2The vitamin premix provided per kilogram of complete diet: 6,614 IU of vitamin A as vitamin A acetate, 992 IU of vitamin D3, 19.8 IU of vitamin E, 2.64 mg of vitamin K as menadione sodium bisulfate, 0.03 mg of vitamin B12, 4.63 mg of riboflavin, 18.52 mg of D-pantothenic acid as calcium panthonate, 24.96 mg of niacin, and 0.07 mg of biotin.
3The trace mineral premix provided per kilogram of complete diet: 4.0 mg of Mn as manganous oxide, 165 mg of Fe as ferrous sulfate, 165 mg of Zn as zinc sulfate, 16.5 mg of Cu as copper sulfate, 0.30 mg of I as ethylenediamine dihydroiodide, and 0.30 mg of Se as sodium selenite.
4The analyzed compositions of DM and CP for phase 1 and 2 were determined by averaging the values obtained from the analyzed levels of the 4 diets in each respective phase.



View Full Table | Close Full ViewTable 2.

Growth performance of nursery pigs fed supplemental nucleotides for 28 d

 
Treatment1
P-value
Item 0.0 0.2 0.5 1.0 SEM Linear Quadratic Cubic
Age at wean, d 21.9 21.8 22.2 22.6 0.3 0.046 0.653 0.634
BW, kg
    d 0 7.25 7.27 7.27 7.27 0.07 0.887 0.824 0.927
    d 0 to 7 7.46 7.66 7.66 7.95 0.12 0.008 0.978 0.428
    d 7 to 14 8.93 8.70 8.91 8.88 0.21 0.894 0.820 0.408
    d 14 to 21 11.40 11.30 11.53 11.82 0.27 0.178 0.704 0.678
    d 21 to 28 15.10 14.87 15.43 16.03 0.37 0.035 0.603 0.489
ADG, g/d
    d 0 to 7 25.7 45.6 43.5 83.7 14.3 0.007 0.658 0.417
    d 7 to 14 210.2 148.2 178.0 136.9 28.5 0.160 0.776 0.170
    d 14 to 21 353.2 371.4 374.8 420.6 23.3 0.043 0.752 0.677
    d 21 to 28 528.3 510.5 556.4 600.4 32.0 0.053 0.679 0.507
    d 7 to 28 363.9 343.4 369.7 386.0 16.0 0.148 0.549 0.294
    d 0 to 28 270.9 261.2 279.1 301.8 12.4 0.031 0.499 0.465
ADFI, g/d
    d 0 to 7 150.9 193.7 172.8 197.1 8.6 0.005 0.406 0.002
    d 7 to 14 288.8 266.0 281.1 279.3 11.2 0.913 0.533 0.187
    d 14 to 21 526.2 542.0 538.5 605.6 13.6 <0.001 0.161 0.315
    d 21 to 28 721.4 766.3 792.1 879.3 18.7 <0.001 0.888 0.439
    d 7 to 28 512.1 524.8 537.3 588.0 9.5 <0.001 0.317 0.642
    d 0 to 28 412.5 433.5 436.7 480.2 7.8 <0.001 0.525 0.174
G:F, g/g
    d 0 to 7 0.014 0.268 0.232 0.401 0.098 0.015 0.548 0.206
    d 7 to 14 0.702 0.507 0.611 0.439 0.098 0.128 0.956 0.182
    d 14 to 21 0.674 0.686 0.702 0.701 0.042 0.640 0.783 0.957
    d 21 to 28 0.730 0.666 0.690 0.688 0.036 0.639 0.459 0.341
    d 7 to 28 0.712 0.655 0.684 0.657 0.026 0.300 0.679 0.179
    d 0 to 28 0.656 0.602 0.637 0.629 0.025 0.800 0.537 0.158
1Diets were supplemented with 0.0, 0.2, 0.5, or 1.0 g/kg of a nucleotide additive (Inotide; CJ Corp, Seoul, Korea). Pigs were fed a phase 1 diet for the first 7 d followed by a phase 2 diet from d 7 to 28.



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Plasma immune and oxidative stress parameters of nursery pigs fed supplemental nucleotides for 28 d

 
Treatment2
P-value
Item1 0.0 0.2 0.5 1.0 SEM Linear Quadratic Cubic
Phase 12
    IgA, mg/mL 0.15 0.14 0.13 0.14 0.01 0.757 0.179 0.903
    IgG, mg/mL 7.89 7.59 6.23 7.67 1.65 0.885 0.493 0.733
    IgM, mg/mL 0.82 0.74 0.80 0.83 0.10 0.717 0.722 0.581
    TNFα, pg/mL 94.42 91.17 81.67 89.92 7.45 0.620 0.297 0.652
    8-OHdG, ng/mL 1.44 1.65 1.29 1.29 0.14 0.204 0.959 0.134
Phase 22
    IgA, mg/mL 0.63 0.48 0.48 0.63 0.06 0.657 0.019 0.489
    IgG, mg/mL 6.32 6.61 6.43 7.00 1.25 0.724 0.921 0.850
    IgM, mg/mL 1.55 1.95 1.39 1.80 0.19 0.770 0.556 0.036
    TNFα, pg/mL 126.67 99.58 98.17 85.58 14.99 0.093 0.508 0.481
    8-OHdG, ng/mL 0.54 0.40 0.36 0.41 0.06 0.157 0.064 0.576
1Phase 1 concentrations measured in plasma collected on d 7, and phase 2 concentrations measured in plasma collected on d 28. TNFα = tumor necrosis factor-alpha; 8-OHdG = 8-hydroxy-deoxyguanosine.
2Diets supplemented with 0.0, 0.2, 0.5, or 1.0 g/kg of a nucleotide additive (Inotide; CJ Corp, Seoul, Korea).



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Fecal scores as a measure of diarrhea of nursery pigs fed supplemental nucleotides for 28 d

 
Treatment1
P-value
Item 0.0 0.2 0.5 1.0 SEM Linear Quadratic Cubic
Fecal score2
    d 5 1.07 1.11 1.11 1.15 0.09 0.555 0.949 0.835
    d 8 1.33 1.12 1.03 1.26 0.12 0.870 0.072 0.870
    d 15 1.48 1.26 1.35 1.45 0.15 0.843 0.386 0.471
1Diets supplemented with 0.0, 0.2, 0.5, or 1.0 g/kg of a nucleotide additive (Inotide; CJ Corp, Seoul, Korea).
2Fecal scores were determined based on the following scoring values: 1 = normal, firm feces, 1.5 = looser feces leading to possible slight diarrhea, 2 = moderate liquid consistency, 2.5 = definite diarrhea with unformed, fluid feces, or 3 = severe diarrhea with very watery and frothy feces. d 5: during phase 1, d 8: after the transition from a phase 1 to 2 diet, and d 15: during consumption of the phase 2 diet.