Fecal concentrations of total Escherichia coli (open bars) in relation to Escherichia coli O157 prevalence (solid bars) in feces collected at h 0, 5, and 29 from cattle subjected, or not, to 1-h transport. Transported animals (10 steers) were placed into a trailer and transported for 1 h. Upon return to the research facility, animals were unloaded into new, clean concrete-surfaced pens. Nontransported animals (10 steers) remained in their pens at all times throughout the experiment. Fecal samples were taken from each animal of each group pretransport (h 0), and at 4 and 28 h post-transport (h 5 and h 29) from freshly voided fecal pats. The experiment was repeated 3 times using a new set of animals for each replication. E. coli O157 prevalence effect on total E. coli, P = 0.954.