Figure 1.
Figure 1.

Effect of saline (SAL) or trace mineral injection (Multimin90 [MM]) on plasma Se (A) and Mn concentrations (B); values are means ± SEM, n = 20 per injection treatment; injection (P < 0.01) and injection × time interaction (P < 0.01). Asterisks (*) denote differences (P < 0.05) between treatments within day of experiment.

 


Figure 2.
Figure 2.

Effect of supplemental trace minerals (control [CON]) or deprivation (deficient [DEF]) during the depletion period and saline (SAL) or trace mineral injection (Multimin90 [MM]) on plasma Zn concentrations; values are means ± SEM, n = 10 within each treatment × diet combination. There was a diet × day interaction (P = 0.01) and injection × day interaction (P < 0.05); asterisks (*) denote differences (P < 0.05) between injections and daggers (†) denote differences (P < 0.10) between depletion diets, within day of the experiment.

 


Figure 3.
Figure 3.

Effect of saline (SAL) or trace mineral injection (Multimin90 [MM]) on red blood cell lysate (RBCL) manganese-superoxide dismutase (Mn-SOD) activity; values are means ± SEM, n = 20 per injection treatment; injection (P = 0.01). Hb = hemoglobin.

 


Figure 4.
Figure 4.

Effect of supplemental trace minerals (control [CON]) or deprivation (deficient [DEF]) during the depletion period and saline (SAL) or trace mineral injection (Multimin90 [MM]) on liver Cu (A), Mn (B), and Se concentrations (C); values are means ± SEM, n = 10 within each treatment × diet combination. Within liver Cu there was a diet × day interaction (P < 0.05) and injection × day interaction (P < 0.05); asterisks (*) denote differences (P < 0.05) between injections and daggers (†) denote differences (P < 0.10) between depletion diets, within day of the experiment. Within liver Mn there was a diet × day interaction (P < 0.001); asterisks (*) denote differences (P < 0.05) between depletion diets. Within liver Se there was a diet × injection × day interaction (P = 0.02); lowercase letters that differ denote differences (P < 0.05) between diet and injection within day.