Figure 1.
Figure 1.

Ruminal fluid and ruminal gas cap sampling apparatus. A, cannula plug (1EZ Easy-out Stopper, Bar Diamond Inc., Parma, ID); B, ruminal fluid collection tube (Bar Diamond, reference 33055, DESC-1); C, 102-mm 14-gauge needle; D, 8-cm-long (4.8-mm-diam.) tubing; E, plastic tubing clamp; F, female luer lock catheter end; G, Argyle intermittent infusion plug (Covidien, Saint-Laurent, QB, Canada, reference 115006); H, 140-mL syringe used for ruminal fluid collection; I, 140-mL syringe used for rumen gas cap collection; J, 3-way stopcock (Smith Medical, Markham, ON, Canada, reference MX5311l); K, 15-cm-long (4.8-mm-diam.) tubing used for sucking the gas from the rumen; L, 15-cm-long (4.8-mm-diam.) tubing used for sucking the gas from the syringe to the pump; M, gas detection pump (model AP-20S, Sensidyne, Clearwater, FL); N, precision hydrogen sulfide gas detector tube (Sensidyne, number 120SM or 120SF); O, 6-cm-long (4.8-mm-diam.) tubing with female luer lock catheter end used for connecting detector tube with the tubing of syringe. See online version for figure in color.

 


Figure 2.
Figure 2.

The effects of dietary sulfur concentration (high S [HS] vs. low S [LS]) and forage-to-concentrate ratio (low F:C [LF:C] vs. high F:C [HF:C]) on DMI (kg/d) of beef heifers. Effects of dietary S (P < 0.001), F:C (P = 0.26), their interaction (P = 0.87), week of sampling (P = 0.002), week × S interaction (P = 0.66), week × F:C interaction (P = 0.15), and week × S × F:C interaction (P = 0.34). Error bars represents SEM.

 


Figure 3.
Figure 3.

The effects of dietary sulfur concentration (high S [HS] vs. low S [LS]) and forage-to-concentrate ratio (low F:C [LF:C] vs. high F:C [HF:C]) on S intake (g/d) of beef heifers. Effects of dietary S (P < 0.001), F:C (P = 0.53), their interaction (P = 0.52), week of sampling (P = 0.003), week × S interaction (P = 0.97), week × F:C interaction (P = 0.12), and week × S × F:C interaction (P = 0.36). Error bars represents SEM.