View Full Table | Close Full ViewTable 1.

Crude protein, fiber, and plant secondary compound content (mean ± SD, DM basis) of the forages and supplements offered to lambs during the study

 
Item CP, g/100 g ADF, g/100 g NDF, g/100 g CT,1 g/100 g Ergovaline, µg/kg
Pen trial – conditioning period
    E+2 11.5 ± 1.17 40.8 ± 4.58 59.6 ± 6.40 100.2 ± 10.74
    Sainfoin subperiod 1 13.2 ± 1.28 41.8 ± 3.91 48.7 ± 4.99 7.7 ± 0.53
    Sainfoin subperiod 2 15.7 ± 1.87 29.9 ± 2.71 36.5 ± 4.68 3.0 ± 0.27
    Beet pulp 9.8 ± 1.02 26.2 ± 3.20 38.1 ± 3.63
    Alfalfa pellets 15.6 ± 1.61 30.1 ± 3.77 41.0 ± 3.66
Pen trial – preference between E–3 and orchardgrass
    E– 9.4 ± 0.97 42.9 ± 5.31 61.7 ± 5.51 Nondetectable
    Orchardgrass 12.4 ± 1.57 40.3 ± 4.29 55.3 ± 5.67
Grazing trial
    E+ 12.0 ± 1.46 40.8 ± 4.01 60.2 ± 6.78 170.4 ± 19.15
    Sainfoin 13.2 ± 1.34 41.2 ± 3.77 48.2 ± 4.48 7.6 ± 0.51
1CT = condensed tannins.
2E+ = endophyte-infected tall fescue.
3E– = endophyte-free tall fescue.



View Full Table | Close Full ViewTable 2.

Complete blood cell count of lambs fed endophyte-infected tall fescue and supplemented with beet pulp (CTRL), fresh-cut sainfoin plus polyethylene glycol mixed in beet pulp (SAIN+PEG), or fresh-cut sainfoin and beet pulp (SAIN)

 
Item Treatment
P-value Ref.1
CTRL SAIN+PEG SAIN SEM
RBC2 (× 106/μL) 13.1 12.5 13.1 0.18 0.065 9–15
Hemoglobin, g/dL 12.6a 12.2b 12.9a 0.12 0.006 9–15
Packed cell volume, % 34.7ab 33.8b 35.3a 0.45 0.046 27–45
Mean cell volume, fL 26.6 26.4 26.5 0.16 0.710 28–40
MCHC,i3 g/dL 36.7 36.6 37.1 0.22 0.343 31–34
RDW4 18.4a 18.4a 17.9b 0.11 0.035
Leukocytes (× 103/μL) 9.0c 9.6b 10.3a 0.17 <0.001 4–12
Lymphocytes (× 103/μL) 6.1b 6.6ab 7.3a 0.22 0.033 2–9
Neutrophils (× 103/μL) 2.4 2.2 2.2 0.17 0.389 0.7–6
Monocytes (× 103/μL) 0.29 0.37 0.38 0.063 0.565 0–0.75
Basophils (× 103/μL) 0.09 0.07 0.06 0.015 0.255 0–0.3
Eosinophils (× 103/μL) 0.11 0.11 0.10 0.021 0.778 0–1
Platelets (× 103/μL) 461.3 522.0 481.1 24.74 0.186 250–750
Mean platelet volume 6.9 6.6 6.7 0.12 0.147
N:L5 0.39a 0.33b 0.30b 0.010 0.007
a,b,cWithin a row, means without a common superscript differ (P < 0.05).
1Ref. = reference; reference range values were taken from Merck (2012a).
2RBC = red blood cells.
3MCHC = mean corpuscular hemoglobin concentration.
4RDW = red blood cell distribution width.
5N:L = neutrophil:lymphocyte ratio.



View Full Table | Close Full ViewTable 3.

Blood serum biochemistry and hormonal profile of lambs fed endophyte-infected tall fescue and supplemented with beet pulp (CTRL), fresh-cut sainfoin plus polyethylene glycol mixed in beet pulp (SAIN+PEG), or fresh-cut sainfoin and beet pulp (SAIN)

 
Item Treatment
SEM P-value Ref.1
CTRL SAIN+PEG SAIN
ALP,2 U3/L 208.8a 168.3b 165.8b 11.55 0.026 27–156
AST,4 U/L 57.0b 65.9a 69.0a 2.76 0.015 49–123
GGT,5 U/L 43.2b 45.2ab 47.5a 0.95 0.017 20–44
Creatine kinase, U/L 93.5 121.7 123.0 9.65 0.054 7.7–101
BUN,6 mg/100 mL 6.5c 12.7a 10.5b 0.80 <0.001 10–26
Creatinine, mg/100 mL 0.83 0.77 0.79 0.024 0.157 0.9–2.0
Total protein, g/100 mL 5.5b 5.7ab 5.9a 0.08 0.006 5.9–7.8
Albumin, g/100 mL 2.9 2.9 3.0 0.03 0.743 2.7–3.7
Globulin, g/100 mL 2.6b 2.8ab 2.9a 0.07 0.009 3.2–5.0
A:G7 1.1a 1.1ab 1.0b 0.02 0.010
Total bilirubin, mg/100 mL 0.13 0.12 0.13 0.012 0.755 0.0–0.5
Triglycerides, mg/100 mL 15.9 16.1 16.2 1.42 0.984
Cholesterol, mg/100 mL 36.4 41.5 37.7 1.53 0.052 44–90
Prolactin, pg/mL 139.3b 180.5ab 210.5a 17.33 0.019
Cortisol, nmol/L 22.8a 13.1b 19.3ab 2.56 0.008
a,b,cWithin a row, means without a common superscript differ (P < 0.05).
1Reference range values were taken from Merck (2012c).
2ALP = alkaline phosphatase.
3U = Enzyme unit.
4AST = aspartate aminotransferase.
5GGT = gamma-glutamyl transpeptidase.
6BUN = blood urea nitrogen.
7A:G = albumin:globulin ratio.