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Diet components and calculated and analyzed contents of the experimental diets (as-fed basis)

 
Experimental diet1
Ingredient MS MD
Corn 7.8% CP 58.25 58.40
Soy bean meal 46.7% CP 35.40 35.40
Soy oil 2.40 2.50
Salt 0.40 0.40
Calcareous 38% 1.34 1.34
Dicalcium phosphate 20% 1.33 1.33
dl-Methionine 99% 0.27
l-Lysine HCl 78% 0.15 0.15
l-Treonine 78% 0.06 0.06
Premix2 0.40 0.4
Total 100.00 100.00
Composition analysis, %
    CP 21.16 21.02
    Lysine digestible 1.15 1.15
    Met + Cis digestible 0.84 0.57
    Treonine digestible 0.78 0.78
    Triptophane digestible 0.23 0.23
    Valine digestible 0.90 0.90
    Isoleucine digestible 0.83 0.83
    Arginine digestible 1.33 1.33
Composition calculated,%
    Ca 0.90 0.90
    P 0.41 0.41
    Na 0.18 0.18
    AME,3 kcal/kg 2,999.06 3,000.08
1MD = methionine deficient; MS = methionine supplementation.
2Supplied by kilogram of diet: retinyl-acetate, 3.44 mg; cholecalciferol, 50 μg; DL-α-tocopherol, 15 mg; thiamine, 1.63 mg; riboflavin, 4.9 mg; pyridoxine, 3.26 mg; cyanocobalamin, 12 μg; D-pantothenic acid, 9.8 mg; D-biotin, 0.1 mg; menadione, 2.4 mg; folic acid, 0.82 mg; niacinamide, 35 mg; selenium, 0.2 mg; iron, 35 mg; copper, 8 mg; manganese, 60 mg; Zn, 50 mg; I, 1 mg; choline, 650 mg; salinomycin, 60 mg; avilamycin, 5 mg; and butyl hydroxy toluene, 80 mg.
3AME = apparent metabolizable energy.



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Daily weight gain (DWG) and proportional weight of organs and muscles

 
Diet - Environment Diet × Environment DWG, g
Proportional weight (%)
Liver
Abdominal fat
Breast
Legs
Mean SD Mean SD Mean SD Mean SD Mean SD
MS1 Comfort 1.61 0.079 1.10 0.19 2.21 0.50 25.92 1.79 14.96 0.44
Stress 0.31 0.036 0.91 0.39 1.01 0.51 25.79 0.97 14.96 0.53
MD2 Comfort 1.65 0.094 1.18 0.40 1.60 0.40 24.84 1.83 15.31 0.21
Stress –0.23 0.023 0.88 0.35 0.58 0.20 24.69 1.44 14.94 0.39
Main effects
    Diet MS 1.17 0.41 1.01 0.30 1.61a 0.79 25.85 1.33 14.96 0.45
MD 1.00 0.36 1.03 0.39 1.10b 0.62 24.77 1.53 15.12 0.35
    Environment Comfort 1.63a 0.41 1.14 0.29 1.91a 0.52 25.38 1.77 15.13 0.37
Stress 0.04b 0.004 0.90 0.34 0.80b 0.42 25.24 1.28 14.95 0.43
Probabilities
    Diet NS3 NS 0.0319 NS NS
    Environment <0.0001 NS 0.0002 NS NS
    Interaction NS NS NS NS NS
a,bMean values within a row with unlike superscript letters were significantly different (P < 0.05).
1MS =methionine supplementation.
2MD = methionine deficient.
3NS = Not significant.



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Analysis of oxidative stress markers and antioxidant enzyme activities in the liver

 
GSH1
TBARS2
GP-x3 activity
Cat4 activity
Diet - Environment Diet × Environment Mean SD Mean SD Mean SD Mean SD
MS5 Comfort 13.70 3.23 7.57b 2.15 172.46bc 8.08 29.91c 4.80
Stress 10.15 0.37 9.63b 1.78 245.72a 37.63 89.16b 12.11
MD6 Comfort 9.12 1.22 6.15b 1.12 162.54c 18.04 31.66c 3.33
Stress 8.63 2.28 13.14a 3.01 195.23b 14.91 203.92a 34.56
Main effects
    Diet MS 11.92a 2.86 9.10 1.62 209.09 36.23 59.54 7.82
MD 8.87b 1.75 10.14 2.67 178.88 23.24 117.79 23.08
    Environment Comfort 11.41a 3.33 7.36 3.85 167.49 14.28 30.79 4.04
Stress 9.39b 1.74 11.39 2.42 220.47 37.95 146.54 21.06
Probabilities
    Diet 0.0273 NS7 0.0037 <0.0001
    Environment 0.0018 <0.0001 <0.0001 <0.0001
    Interaction NS 0.0386 0.0392 <0.0001
a–cMean values within a row with unlike superscript letters were significantly different (P < 0.05).
1GSH = glutathione. Expressed as μg/mg of protein.
2TBARS = thiobarbituric acid reactive substances. Expressed as nmol TBARS/mg of protein.
3GP-x = glutathione peroxidase. Expressed as nmol of oxidized nicotinamide adenine dinucleotide phosphate /mg of protein per minute.
4Cat = catalase. Expressed as units/mg of protein.
5MS = methionine supplementation.
6MD = methionine deficient.
7NS= Not significant.



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Plasma analysis of uric acid, creatinine, and creatine kinase (CK) activity levels

 
Uric acid, mg/dL
Creatinine, mg/dL
CK activity, units/L
Diet - Environment Diet × Environment Mean SD Mean SD Mean SD
MS1 Comfort 6.13b 1.41 0.20c 0.0002 2,707.83a 138.75
Stress 10.52a 2.26 0.23b 0.0052 2,377.00a 176.99
MD2 Comfort 6.43b 1.93 0.20c 0.0003 2,068.33a 150.40
Stress 5.42b 0.70 0.30a 0.002 1,620.33b 203.68
Main effects
    Diet MS 8.32 2.91 0.20 0.0001 2,388.10 176.11
MD 5.93 1.48 0.27 0.049 1,989.70 131.44
    Environment Comfort 6.28 1.62 0.22 0.04 2,542.04 183.18
Stress 7.97 3.10 0.25 0.05 1,844.30 173.05
Probabilities
    Diet 0.0023 <0.0001 NS3
    Environment 0.0237 0.0049 0.0347
    Interaction 0.0008 0.0049 0.0442
a–cMean values within a row with unlike superscript letters were significantly different (P < 0.05).
1MS = methionine supplementation.
2MD = methionine deficient.
3NS= Not significant.