Expression of the bovine Sirt1 protein by resveratrol (RSV) and lipoic acid (LA) treatments. Western blot analysis was used to determine the expression of the Sirt1 protein in liver (A) and muscle (B). Rat liver (+) was used as the positive control for both tissues (upper panel). Quantification of the Sirt1 protein expression was made relative to ACTB using the ImageJ software (National Institutes of Health, Bethesda, MD; lower panel). Data from 6 animals are presented as the least squares means ± SEM. Different lowercase letters indicate a significant difference between treatments under the same condition (P < 0.05). Treatments are as follows: Control (bars 1 and 8), 40 μM RSV (bars 2 and 9), 80 μM RSV (bars 3 and 10), 30 μM LA (bars 4 and 11), 100 μM LA (bars 5 and 12), 300 μM LA (bars 6 and 13), and 1,000 μM LA (bars 7 and 14).