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Primer sequences for real-time PCR

 
Gene1 Forward Reverse
CD36 5′–ATC GTG CCT ATC CTC TGG–3′ 5′–CCA GGC CAA GGA GGT TAA–3′
FATP4 5′–CAT TGT GGC TCA GCA GGT TA–3′ 5′–CAG GCT AGG GGT CAA ATC AA–3′
C/EBPα 5′–TGG ACA AGA ACA GCA ACG AG–3′ 5′– TTG TCA CTG GTC AGC TCC AG–3′
aP2 5′–TGG TAC AGG TGC AGA AGT GG–3′ 5′–ATT CTG GTA GCC GTG ACA CC–3′
HSP70 5′–TTC GTG GAC AGA AGC CAC AG –3′ 5′–TTG CTA GGA TCT CCA CCC GA–3′
FAS 5′–AGT TTG TGA TGG AGA ACA CGG CCT–3′ 5′–TGT TCA CAC GTG GTG CAA GGG TTA–3′
GK 5′–CGC TGA GGA AAG TGA AAT CCG–3′ 5′–TCG CGT CTT TGG AAT CTA CGA–3′
PCK1 5′–CCC TGC CTT TGA AAA AGC CC–3′ 5′–GGA GAT GAT TTC TCG GCG GT–3′
18S 5′–ATC CCT GAG AAG TTC CAG CA–3′ 5′–CCT CTT GGT GAG GTC GAT GT–3′
PPARγ 5′–GCC CTT CAC CAC TGT TGA TT–3′ 5′–GTT GGA AGG CTC TTC GTG AG–3′
LPL 5′–ATT CAC CAG AGG GTC ACC TG–3′ 5′–AGC CCT TTC TCA AAG GCT TC–3′
Leptin 5′–TTG GCC CTA TCT GTC CTA CG–3′ 5′–GTG ACC CTC TGT TTG GAG GA–3′
HSP27 5′–GAG CTG ACG GTC AAG ACC AA–3′ 5′–AAT GAA GCC GTG CTC ATC CT–3′
HSP90 5′–GTC GAA AAG GTG GTT GTG TCG–3′ 5′–TTT GCT GTC CAG CCG TAT GT–3′
GLUT4 5′–GAA GGA AGA AGG CAA TGC TG–3′ 5′–GAG GAA CCG TCC AAG AAT GA–3′
Adiponectin 5′–TTT CTG GGC CCA CTG TGT TT–3′ 5′–GGT TTT GCA TTG CAG GCT CA–3′
HSP60 5′–AGA TGC CCT GAA TGC GAC AA–3′ 5′–TGA CTC CAA GGC TGG AAT GC–3′
FATP6 5′–TTC TTC GGCTAT GCT GGC AA–3′ 5′–TGG ACC ATTAGG TCT CCG GT–3′
1aP2 = fatty acid binding protein 2; CD36 = fatty acid translocase 36; C/EBP α = CCAAT/enhancer binding proteins α; FATP4 = fatty acid transport protein 4; FATP6 = fatty acid transport protein 6; FAS = fatty acid synthase; GLUT4 = glucose transporter protein type 4; GK = glycerol kinase; HSP = heat shock protein; LPL = lipoprotein lipase; PCK1 = phosphoenolpyruvate carboxykinase 1; PPARγ = peroxisome proliferator-activated receptor γ.