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Composition and analysis of the experimental diets

 
Diet1 CON 15WECS 30WECS
Ingredient, % DM
    Corn meal 47.1 36.1 25.1
    Barley meal 23.1 23.1 23.1
    Wheat meal 10.0 10.0 10.0
    Soya bean meal, extracted 9.0 9.0 9.0
    Wheat bran 8.0 4.0 0.0
    Whole ear corn silage 0.0 15.0 30.0
    NaCl 0.36 0.36 0.36
    CaCO3 1.13 1.13 1.13
    CaHPO4 0.75 0.75 0.75
    L-lys HCL 0.09 0.09 0.09
    Supplement2 0.47 0.47 0.47
    DM, % 91.5 86.5 81.8
    CP, % DM 14.0 13.7 13.6
    NDF, % DM 15.8 15.4 15.2
    ADF, % DM 4.9 5.2 5.5
    Ether extract, % DM 2.7 2.7 2.6
    Ash, % DM 5.5 4.8 4.5
    Starch % DM 47.5 48.3 49.1
1CON, control diet with 0% whole-ear corn silage; 15WECS, diet with 15% whole-ear corn silage of DM; 30WECS, diet with 30% whole-ear corn silage of DM.
2 Supplied per kilogram DM of complete diet: vitamin A, 9400 IU; vitamin D3, 1880 IU; vitamin E, 47 mg; vitamin K3, 1.0 mg; thiamine, 0.9 mg; riboflavin, 4.7 mg; niacin, 23.5 mg; vitamin B6, 0.9 mg; vitamin B12, 0.024 mg; pantothenic acid, 12 mg; biotin, 0.19 mg; choline chloride, 118 mg; Fe, 126 mg from FeSO4.H2O; Cu, 21 mg from CuSO4×5H2O; Zn, 127 mg from ZnO; Mn, 42 mg from MnO2; I, 0.7 mg from Ca(IO3)2; Co, 0.2 mg from 2CoCO3 3Co(OH)2×H2O; Se, 0.2 mg from Na2SeO3 (pig supplement 0.5%).



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Effects of different dietary inclusion of whole-ear corn silage (WECS) and the exit from a metabolic cage on hematological profile in barrows; values are reported as LSM

 
Diet1
Housing
P-value
Item CON 15WECS 30WECS Pen Cage SEM or CI2 Diet (D) Sampling (S) D×S Housing
WBC3, k/mL 16.1 16.0 17.9 16.8 16.5 16.0–17.4 0.275 0.002 0.003 0.493
NEU, k/mL 3.45 4.41 4.75 4.25 4.09 3.89–4.55 0.060 0.002 0.398 0.511
LYM, k/mL 10.5 9.4 11.1 10.3 10.2 9.8–10.7 0.106 0.587 0.128 0.864
NEU to LYM, ratio 0.33 0.47 0.43 0.42 0.39 0.38–0.45 0.020 0.343 0.592 0.458
MON, k/mL 1.06 1.11 1.07 1.11 1.05 1.01–1.18 0.921 0.053 0.760 0.356
EOS, k/mL 0.57 0.38 0.49 0.51 0.44 0.44–0.54 0.071 0.220 0.098 0.062
BAS, k/mL 0.22 0.31 0.21 0.24 0.25 0.22–0.27 0.144 0.003 0.515 0.830
RBC, M/mL 6.88 6.84 7.05 6.55 7.29 0.07 0.456 <0.001 0.987 <0.001
Hgb, g/dL 13.4 13.2 13.6 12.7 14.2 0.13 0.519 0.001 0.994 <0.001
HCT, % 39.8 38.8 40.5 37.5 41.9 0.41 0.211 <0.001 0.948 <0.001
MCV, fL 58.0 56.8 57.6 57.3 57.6 0.50 0.621 <0.001 0.019 0.065
MCH, pg 19.6 19.4 19.3 19.4 19.4 0.18 0.771 0.108 0.051 0.796
MCHC, g/dL 33.8 34.1 33.6 33.8 33.8 0.09 0.041 0.005 0.025 0.982
RDW, % 21.8 21.8 22.0 21.6 22.1 0.23 0.951 0.121 0.518 0.101
PLT, k/mL 295 360 286 340 288 15.0 0.086 0.422 0.109 0.011
MPV, fL 11.5 11.2 12.3 12.2 11.1 0.34 0.416 0.131 0.001 0.008
PCT, % 0.34 0.42 0.36 0.40 0.34 0.02 0.220 0.306 0.051 0.027
1CON, control diet with 0% WECS; 15WECS, diet with 15% WECS on a DM basis; 30WECS, diet with 30% WECS on a DM basis.
2The SEM is pooled and the CI applies to variables not normally distributed.
3WBC, total white blood cells; NEU, neutrophils; LYM, lymphocytes; MON, monocytes; EOS, eosinophils; BAS, basophils; RBC, total red blood cells; Hgb, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RDW, width of RBC volume distribution; PLT, total platelet number; MPV, mean platelet volume; PCT, plateletcrit.



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Effects of different dietary inclusion of whole ear corn silage (WECS) and the exit from a metabolic cage on metabolic profile in barrows; values are reported as LSM

 
Diet2
Housing
P-value
Item1 CON 15WECS 30WECS Pen Cage SEM or CI3 Diet (D) Sampling (S) D×S Housing
Glucose, mmol/L 4.30 4.47 4.22 4.19 4.46 0.07 0.269 0.008 0.174 0.010
Cholesterol, mmol/L 2.46 2.29 2.30 2.33 2.37 0.06 0.466 0.091 0.082 0.233
Triglycerides, mmol/L 0.29 0.29 0.24 0.28 0.27 0.02 0.360 0.094 0.411 0.635
NEFA, mmol/L 0.052b 0.087a 0.079a 0.078 0.065 0.065–0.086 0.050 <0.001 0.243 0.056
Glucose to NEFA, ratio 78.8 62.9 43.5 53.9 66.6 48.7–65.6 0.106 <0.001 0.103 0.033
NEFA to cholesterol, ratio 21.6 38.4 34.0 33.9 27.3 27.9–37.5 0.046 <0.001 0.266 0.024
NEFA to albumin, ratio 1.42 2.40 2.10 2.19 1.70 1.81–2.38 0.045 <0.001 0.169 0.007
BHBA, mmol/L 0.049 0.049 0.041 0.045 0.048 0.007 0.843 0.150 0.121 0.847
Urea, mmol/L 4.44 4.41 3.94 4.24 4.29 0.10 0.079 0.081 0.517 0.744
Creatinine, μmol/L 168 161 169 163 169 3.5 0.600 <0.001 0.178 0.187
Total protein, g/L 73.8 73.6 72.7 71.2 75.5 0.72 0.792 0.148 0.885 <0.001
Albumin, g/L 37.2 36.6 37.2 35.8 38.2 0.48 0.812 0.568 0.865 <0.001
Globulins, g/L 36.7 37.0 35.5 35.5 37.3 0.59 0.532 0.019 0.689 0.005
Albumin to globulins, ratio 1.02 1.00 1.06 1.02 1.03 0.02 0.473 0.018 0.557 0.565
Ca, mmol/L 2.62 2.64 2.63 2.60 2.67 0.02 0.922 <0.001 0.333 0.002
P, mmol/L 2.60 2.58 2.64 2.67 2.55 0.02 0.453 0.003 0.814 0.005
Mg, mmol/L 0.80 0.78 0.81 0.79 0.81 0.01 0.436 0.316 0.818 0.350
Na, mmol/L 144 146 144 144 146 0.38 0.164 0.305 0.655 0.002
K, mmol/L 5.14 4.97 5.14 5.05 5.12 0.05 0.204 0.456 0.835 0.416
Cl, mmol/L 103 102 101 102 102 0.29 0.149 0.820 0.093 0.240
Fe, μmol/L 24.8 24.0 21.6 20.7 26.2 0.59 0.074 0.211 0.161 <0.001
Ca to P, ratio 1.01 1.03 1.00 0.98 1.05 0.01 0.529 0.100 0.508 <0.001
CAB, mEq/L 46.9 48.4 48.1 46.9 48.7 0.30 0.105 0.441 0.912 <0.001
Amylase, U/L 1896 2152 1805 1915 1987 103 0.370 0.013 0.782 0.071
ALT, U/L 47.1 44.3 46.3 44.8 47.0 1.34 0.671 0.019 0.786 0.210
AST, U/L 38.2 34.9 43.4 40.0 37.6 1.94 0.177 0.740 0.583 0.578
GGT, U/L 39.6 34.1 37.5 37.5 36.6 1.79 0.449 <0.001 0.128 0.481
Total bilirubin, μmol/L 1.20 1.31 1.19 1.28 1.18 0.06 0.632 0.805 0.037 0.475
ALP, U/L 125 112 121 127 112 4.6 0.521 0.032 0.503 0.023
TRAP, U/L 5.76 6.51 5.96 6.52 5.64 0.26 0.425 0.892 0.888 0.066
LDH, U/L 747b 769b 947a 770 865 741–839 0.005 0.071 0.822 0.114
1BHBA, D-3-Hydroxybutyrate; ALT, alanine aminotransferase; AST, aspartate aminotransferase; ALP, alkaline phosphatase; GGT, γ-glutamyltransferase; LDH, L-lactate dehydrogenase; TRAP, tartrate-resistant acid-phosphatase; CAB, plasma cation-anion balance.
2CON, control diet with 0% WECS; 15WECS, diet with 15% WECS on a DM basis; 30WECS, diet with 30% WECS on a DM basis.
3The SEM is pooled and the CI applies to variables not normally distributed.
a,bWithin a row, means without common superscripts differ (P < 0.05).



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Effects of different dietary inclusion of whole ear corn silage (WECS) and the exit from a metabolic cage on serum protein profile in barrows; alues are reported as LSM

 
Diet1
Housing
P-value
Item CON 15WECS 30WECS Pen Cage SEM2 Diet (D) Sampling (S) D×S Housing
Albumin, % 48.9 47.9 48.4 48.1 48.7 0.54 0.733 0.330 0.014 0.341
α-globulin, % 19.4 20.2 19.4 19.5 19.8 0.38 0.583 0.484 0.083 0.517
β-globulin, % 11.2 11.5 11.9 12.3 10.8 0.32 0.635 0.326 0.157 0.006
γ-globulin, % 20.6 20.3 20.3 20.1 20.7 0.41 0.966 0.349 0.370 0.125
1CON, control diet with 0% WECS; 15WECS, diet with 15% WECS on a DM basis; 30WECS, diet with 30% WECS on a DM basis.
2The SEM is pooled.