Figure 1.
Figure 1.

Analysis of Mef2B expression patterns in different tissues of 12-mo-old HF bulls. Bars represent the mean ± SE (n = 10). *P < 0.05; **P < 0.01. LM, longissimus dorsi; GM, gluteus medium; ST, semitendinosus.

 


Figure 2.
Figure 2.

Breed-specific (A) and age-dependent (B) expression level of the Mef2B in LM of four cattle breeds. Data are means ± SE (n = 10). *P < 0.05; **P < 0.01; ***P < 0.001. HF, Polish Holstein-Friesian; LIM, Limousine; HER, Hereford; PR, Polish Red.

 


Figure 3.
Figure 3.

Western blot analysis of MEF2B protein level in LM of 4 cattle breeds at 3 stages of postnatal development. (A) Representative blots of MEF2B and GAPDH protein; (B) ratio of relative protein levels of MEF2B expressed to GAPDH. GAPDH was used as a loading control, as indicated. Data are representative for three independent Western blot analyses performed using extracts from 3 bulls of each experimental group. Results are expressed as mean ± SE. Statistically significant differences (**P < 0.01; ***P < 0.001) in MEF2B protein levels were calculated within breed in a given age group. LIM, Limousine; HER, Hereford; HF, Polish Holstein-Friesian; PR, Polish Red.

 


Figure 4.
Figure 4.

The effect of JX065116:g.4546G > A in the 3’UTR on Mef2B mRNA and MEF2B protein abundance in LM of 12-mo-old HF bulls. (A) Relative Mef2B mRNA level in LM of HF bulls representing GG (n = 10), AA (n = 10), and AG (n = 10) genotypes for SNP JX065116:g.4546G > A. Data are shown as the mean ± SE. A statistically significant difference at P < 0.05 was calculated between the GG and AA genotypes. (B) Western blot analysis of the MEF2B protein level in LM of HF bulls with different JX065116:g.4546G > A variants. Representative blots of MEF2B and GAPDH protein. GAPDH is shown as a loading control. (C) Bar graphs shows densitometry quantification of blots from MEF2B normalized to GAPDH. Relative protein abundance differences are shown as densitometry mean ± SE (n = 10). *P < 0.0.