View Full Table | Close Full ViewTable 1.

Hepatic steroid metabolizing enzyme activity in cows before and after initiation1 of dietary treatments

 
Dietary treatment2
Day
P-value
Dependent variable CON SUP SEM Before After SEM Treatment Day Treatment × day
CYP1A3
    (RLU4 ∙ min-1 ∙ mg-1 protein)×104 79.8 90.1 6.4 65.4 104.4 6.4 0.26 <0.01 0.27
    (RLU ∙ min-1 ∙ g-1)×106 48.4 66.5 4.3 43.6 71.3 4.3 <0.01 <0.01 0.06
CYP2C5
    RLU ∙ min-1 ∙ mg-1 protein 3.0 3.0 0.2 2.8 3.2 0.2 0.96 0.15 0.38
    (RLU ∙ min-1 ∙ g-1)×104 18.5 21.8 1.4 18.3 22.0 1.4 0.11 0.07 0.83
CYP3A6
    RLU ∙ min-1 ∙ mg-1 protein 569.0 685.1 39.2 690.3 563.8 39.2 0.04 0.03 0.64
    (RLU ∙ min-1 ∙ g-1)×106 35.8 50.6 3.2 46.8 39.7 3.2 <0.01 0.13 0.68
UGT7
    (RLU ∙ min-1 ∙ mg-1 protein)×105 10.7 13.2 1.4 8.8 15.2 1.4 <0.01 0.20 0.84
    (RLU ∙ min-1 ∙ g-1)×106 68.2 95.8 9.4 57.8 106.2 9.3 <0.01 0.04 0.85
1Initiation of dietary treatments was d 201 of gestation: before = d 187 (±1 d); after = d 221 to 222.
2Maternal diets: CON = control group (consuming basal diet; n = 15); SUP = treatment group (consuming basal diet + dried distillers’ grains plus solubles at 3 g/kg of BW; n = 12).
3CYP1A = cytochrome P450 1A.
4RLU = relative light units.
5CYP2C = cytochrome P450 2C.
6CYP3A = cytochrome P450 3A.
7UGT = uridine 5′-diphospho-glucuronosyltransferase.



View Full Table | Close Full ViewTable 2.

Calving and placental measurements from beef cows fed the control or the control plus supplementation from d 201 to 270 of gestation

 
Dietary treatment1
Variable CON SUP SEM P-value
Gestation length, d 277.0 275.8 1.0 0.43
Calf birth wt, kg 39.8 43.2 1.0 0.02
Calving ease2 1.87 1.44 0.36 0.39
Heart girth,3 cm 82.1 84.1 1.2 0.22
Crown rump length,4 cm 84.7 83.1 2.0 0.57
Calf 24 h wt, kg 40.4 44.0 1.1 0.02
Placental expulsion, min 226.8 286.3 54.5 0.45
Cotyledonary number 90.5 85.5 9.1 0.68
Total placenta wt, kg 5.4 4.7 0.4 0.22
Intercotyledonary wt,5 kg 2.7 2.2 0.2 0.13
Cotyledonary wt,6 kg 2.2 2.2 0.2 0.94
Largest, g 90.1 91.7 9.0 0.90
Smallest, g 1.0 0.5 0.2 0.18
1Maternal diets: CON = control group (consuming basal diet; n = 15); SUP = treatment group (consuming basal diet + dried distillers’ grains plus solubles at 0.3% of BW; n = 12).
2Calving ease score (1 = no assistance and 5 = caesarian section).
3Heart girth was measured around calf’s chest at withers.
4Crown rump measured from the point of the skull to the base of the tail head.
5Intercotyledonary weight includes all tissues except cotyledons.
6Cotyledonary weight includes all dissected cotyledons.